Equipment

Mass spectrometry equipment

 

Waters SynaptTM HDMS

Type: Quadrupole (TWIMS) Time-of-flight
Date acquired: 2008
Comments: Triwave technology with high efficiency ion mobility spectrometry enabling sample separation based on molecule's mass as well as their size, shape and charge
Sources: Electrospray (ESI), nanospray and lockspray
Use: Ion mobility study, time aligned parallel fragmentation for structural analysis, high mass protein and DNA research and accurate mass analysis


Waters Qtof Ultima

Type: Quadrupole Time-of-flight
Date acquired: 2004
Comments: Factory modified for high mass/charge transmission
Sources: Electrospray (ESI), nanoESI, lockspray
Use: High mass protein analysis, binding interactions and DNA research.  Accurate mass analysis


Waters Quattro Micro

Type: Triple Quadrupole
Date acquired: 2004 (as 2nd hand instrument)
Sources: Electrospray (ESI), nanESI
Use: Gas phase thermochemistry, reaction monitoring and lipid research


Waters Xevo QTOF

Type: Quadrupole time-of-flight
Date acquired: 2010
Sources: Electrospray (ESI), Lockspray and Atmospheric Solids Analysis Probe (ASAP)
Use: Accurate mass analysis, high resolution mass spectrometry, tandem mass spectrometry
Additional Features: UPLC-MS and GC-MS


AB SCIEX QTrap 5500 With Selexion

Type: Triple quadrupole and linear ion trap
Date acquired: 2013
Comments: Fitted with selexION differential ion mobility spectrometry (DMS) device to allow separation of isobaric and co-eluting molecules. This instrument is part of a collaboration with AB Sciex (Toronto, Canada) through the ARC Linkage Program and has been modified to measure Ozone Induced Dissociation (OzID) mass spectra. OzID is a technique developed in our lab and uses a selective reaction between the ion and ozone gas to cleave double bonds and reveal their position(s) within the molecule. OzID is used routinely for structural characterisation of lipids
Sources: Electrospray
Uses: DMS separation of isomeric lipids, OzID for identification of fatty acid double bond position
Additional features: Eksigent ekspert? nanoLC400 system


AB SCIEX QTrap 5500

Type: Triple quadrupole and linear ion trap
Date acquired: 2011
Sources: Electrospray, Advion Nanomate (chip-based nanoESI)
Uses: The instrument is part of the UOW lipidomics facility and is used for the identification and quantification of a wide range of lipids in extracts from diverse sources including tissue, tears and cell culture. The automated nanomate source allows the investigation of large sample arrays
Additional features: Liquid extraction surface analysis (LESA) allowing analysis of molecules directly from a surface


Shimadzu LCMS-2010EV

Type: Single quadrupole
Date acquired: 2013
Sources: Electrospray (ESI)


Shimadzu QP5050A

Type: Gas chromatoraphy / single quadrupole
Date acquired: 2003
Sources: Direct insertion electron impact (EI)
Use: Purity testing, low resolution compound identification


Thermo Fisher Scientific LTQ XL

Type: Linear ion trap, with coupled Surveyor LC
Date acquired: 2013
Sources: Ion Max Electrospray, 2D desorption electrospray (DESI), will be moved to this instrument soon if that counts as a source
Use: Analysis and identification of lipids and fatty acids


Thermo Fisher Scientific LTQ Orbitrap XL

Type: Linear ion trap, with orbitrap mass analyser
Date acquired: 2012
Sources: Matrix assisted laser desorption ionisation (MALDI)
Use: Accurate mass analysis of lipids and peptides. Spatial imaging of lipids in tissues

 

 

Nuclear magnetic resonance spectrometers

The School of Chemistry is currently equipped with three NMR Spectrometers:

The NMR facility is primarily used by the research students and staff in the School of Chemistry. NMR services for external researchers can also be provided (reasonable charges apply).

We also have access to:

  • Bruker 600 NMR spectrometer at UNSW
  • Bruker 800 MHz spectrometer at ANU
Further information:

NMR Facility Manager Dr Wilford Lie
Phone: 42 21 3513 (office)
Phone: 42 21 3473 (lab)