Molecular Horizons Second Annual Light Microscopy Image Competition
MicroWorld in Motion Winners (3D image and video)
- 1st Place | Luke McAlary: Liquid Phase Separation of DDX4 protein
- 2nd Place | Benjamin Genenger: Live cell imaging of co-culture skin cancer spheroids
Liquid Phase Separation of DDX4 protein
Live cell imaging of co-culture skin cancer spheroids. The patient-derived skin cancer cell line, UW-CSCC2 (magenta), and dermal fibroblasts (cyan) were seeded in ultra-low attachment plates to facilitate spheroid formation. Z-stacks of live spheroids were taken every 2h45min for 60h. This revealed the formation of a cancer shell around a fibroblastic core. Investigation of other combinations of fibroblasts and cancer cell lines indicate the formation of distinctive spatial architectures for each combination (not shown).