Conditions for Use of the Cryo-Electron Microscope Facility
All publications that in part of or in full arise from the use of the facility will need to include the following text in the acknowledgments section: We wish to thank and acknowledge the use of the University of Wollongong Cryogenic Electron Microscopy Facility at Molecular Horizons under the management of Dr. James C. Bouwer and Interim Directorship of Senior Professor Eileen McLaughlin.
A faculty oversight committee has been formed to periodically review the operations of the facility as well as to approve any extraordinary exceptions to the following rules. The facility manager has the authority to approve daily operational exceptions as needed.
The Cryo-Electron Microscopy Facility is available to UOW faculty, staff and students and also to members of Australian Academic Institutions at established campus approved recharge rates.
The Facility does not have the resources for sample preparation except for negative staining and plunge freezing of particulate samples. Users are expected to determine what preparation techniques are needed for their own specimens and to perform them in their own lab.
The Facility is open from 9 a.m. to 5 p.m., Monday through Friday. Experienced users may use the Facility after hours at the discretion of the Facility Manager and after proper training has been received.
No user will be allowed to operate the microscopes and ancillary equipment without proper training. The Facility Manager will determine if a potential user’s previous training is adequate, and if not, will provide such training. The ancillary equipment includes, but is not limited to, the:
Users are expected to leave the microscopes, the scope rooms, and the wet lab in a condition such that the Facility is ready for the next user. These include but are not limited to:
Properly shutting down the instruments
Straightening up the rooms that were used
Notifying the Facility Manager if there is a problem with the microscopes or any other piece of equipment and writing down the occurrence in the associated logbook
Notifying the Facility Manager when the liquid nitrogen tanks, the dry nitrogen tanks and any other items are emptied
Turning off all lights, including the hallway, and locking all doors when finishing work after hours
No image files should be stored on the microscope computers and no external, file-storage devices (i.e. flash drives, CDs) should be attached to them. Digital files may be transferred to interim storage resources. Users are requested to remove their data from the CryoEM facility storage servers as soon as possible. Data older than two months will be deleted by the Facility Manager without owner notification.
All established safety procedures need to be followed and all hazardous waste is to be properly disposed.
No user should attempt to fix any equipment problems, regardless of how small or insignificant they may appear to be. The Facility Manager’s contact information is posted outside of each microscope room and a 24 hour phone number is listed.
No user should schedule the T-12 microscope for more than two, full days a week during normal working hours. Extra, available, unscheduled sessions may be claimed if the time until the session is less than 24 hours. Exceptions may be given for extenuating circumstances or if a researcher has collaborators who travel from an outside region.
There will be no restrictions on how much time a user can schedule outside of the normal work week but courtesy to other users is expected.
A user will be charged for a scheduled appointment if the user does not show up or delete the appointment from the scheduler. Charging for the session will begin at the scheduled time.
Users who receive microscope and/or ancillary equipment training will be charged both for the microscope time and for the Facility Manager’s time.
A maximum of 12 hours will be charged to a user for a 24 hour day regardless of how long the instrument is used.