Chemical Reactor Engineering
Ethanol
production through continuous fermentation (2006-present)
The interest in biofuels has renewed since the Kyoto Protocol, where many
industrialized countries agreed to reduce their carbon dioxide emissions
and greenhouse gas production.
Renewable fuels
have the potential to greatly reduce Australia's reliance on
expensive imported oil. One of the
proposed fuel alternatives is ethanol, which can be produced from many
renewable sources such as lignocellulosic waste/materials
(Ward and Singh, 2002).
The largest national ethanol fuel industries exist in
Brazil, where almost 50%
of all cars are able to use 100% ethanol fuels and gasoline sold contains
at least 20% ethanol (Reel; 2006).
Ethanol has a number of attractive features as a fuel and
ethanol blends are increasingly being used worldwide;
more than 10 percent of all gasoline sold in the US in 2002 contained ethanol
(United Sates Environmental Protection Agency, 2002).
It is a much cleaner fuel than gasoline, being biodegradable without
having harmful effects on the environment.
It provides high octane at low cost, acting as an alternative
to harmful fuel additives; ethanol blends can be used in gasoline
engines without modifications. Ethanol's high oxygen content reduces
carbon monoxide levels by 25-30\% according to the US EPA
and dramatically reduces emissions of hydrocarbons, a
major contributor to the depletion of the ozone layer.
Many car manufacturing companies (GM, Ford, Chrysler,
Toyota, Honda) are now developing hybrid vehicle that run on an ethanol
mix.
In the US the Energy Independence and Security Act of 2007 (EISA)
required the use of 16 billion gallons of cellulosic biofuels by 2022
(U.S. Department of Energy, 2008).
According to a study by the U.S. Department of Agriculture
bioethanol generates 35% more
energy than it takes to produce
(Shapouri et al 2002).
In addition, it is a renewable fuel as it may be made from plants.
We have investigated the continuous production of ethanol.
The biochemical model used was originally proposed by
Ghommidh et al (1989),
to account for oscillations observed during the continuous fermentation
of ethanol
using Zymononas mobilis, and
extended by
Jarzebski (1992), to explain oscillations
observed during continuous fermentation using
Zymononas mobilis.
Jarzebski estimated parameter values using data obtained from the
continuous fermentation of sugar-cane blackstrap molasses a temperature
of 37oC by
Pergo et al (1985).
The model contains
five variables: the concentration of substrate, product (ethanol)
and biomass (viable cells, non-viable cells and dead cells).
The biomass is Zymomonas mobilis.
The equation for the concentration of dead cells uncouples from the system,
so that there are four equations in a single reactor. The biochemical
kinetic model accounts for both product inhibition and substrate limitation.
We assume that only the feed contains only substrate.
A combination of steady
state analysis and path following methods is used.
The performance of the reaction scheme in one tank is used as benchmark
for comparing the performance of multiple tanks. We have investigated
ethanol production in both a single tank
(Watt et al, 2007a)
and in reactor cascades
(Watt et al, 2007b;
Sidhu et al, 2008;
Watt et al, 2010). In these
investigations there was no recycle in the system.
Our results for a single reactor include:
- We have established the conditions for washout to occur in a
single reactor
(Watt et al (2007a)).
- We have established in a single reactor that the
steady-state diagram contains no hopf
bifurcations if the substrate concentration in the feed is below
108 gl-1, two hopf bifurcations if the substrate
concentration in the feed (S0) is in the range
108 gl-1 < S0 <
122 gl-1 and one hopf bifurcation point if the substrate
concentration in the feed satisfies
122 gl-1 < S0.
(Watt et al (2007a))
- Our analysis shows that although the system can exhibit interesting
behaviour, such as oscillatory states and a period doubling
root to chaos, the
productivity of the system is optimised by operating in a steady-state
regime.
(Watt et al (2007a)).
- The optimal productivity for a single tank was only very weakly
dependent upon the substrate concentration in the feed and was
3.8 gl-1h-1.
(Watt et al; 2007b)
Our results for a cascade of two reactors include:
Our results for a cascade of three reactors include:
- The optimal productivity of a three-reactor cascade with equal residence
times in both reactors is 4.87 gl-1h-1.
This is an increase of 28% compared to the optimal single reactor.
(S0= 160 gl-1).
(Watt et al; 2007b)
Our results for a cascade of four or five reactors:
- With equal residence times in each reactor we found that
increasing the number of tanks in the cascade to four or five
decreased the maximum productivity:
4.285 gl-1h-1 (four reactors) and
3.776 gl-1h-1 (five reactors).
(Watt et al; 2010).
The principal
contribution of our investigations into ethanol productivity
is not in determining the particular increases that can be gained from
a cascade using this particular biochemical mechanism. Instead, the main
contribution is to show
that path following are efficient tools to investigate the performance
of a single reactor, any two-reactor cascade and any reactor cascade
with equal residence times in each reactor.
- The maximum yield obtained in a single stage continuous
Zymomonas fermenter is significantly lower than the yields
currently obtained in the US corn to ethanol industry, which are
currently around 0.48
(Wallace et al; 2005).
(Sidhu et al; 2008).
- In a two stage continuous
fermenter it was possible to obtained
yields higher than 0.48
by having a total residence time of 15.23 hours and having
different residence times in each stage. To obtain the same yield
in a two-reactor cascade having equal residence times in each stage
a total residence time of 16.22 hours was required.
(Sidhu et al; 2008).
- In a three stage continuous
fermenter, with equal residence times in each stage,
it was possible to obtained a yield of 0.48
by having a total residence time of 16.29 hours.
(Sidhu et al; 2008).
- A.B. Jarzebski. (1992). Modelling of
oscillatory behaviour in continuous ethanol fermentation.
Biotechnology Letters, 14(2), 137-142.
http://dx.doi.org/10.1007/BF01026241.
- C. Ghommidh, J. Vaija, S. Bolarinwa and
J.M. Navarro. Oscillatory behaviour of Zymomonas in continuous
cultures: A simple stochastic model. Biotechnology Letters,
2(9), 659-664.
- L. Pergo, J.M.C.D. Dias, L.H. Koshimizu,
M.R.D. Cruz, W. Borzani and M.L.R. Vairo. (1985). Influence of
temperature, dilution rate and sugar concentration on the establishment
of steady-state in continuous ethanol fermentation of molasses.
Biomass 6(3), 247-256.
- M. Reel (2006).
Brazil's road to energy independence. The Washington Post, August 19th.
- Shapouri, H., Duffield, J.A., and Wang, M.
The energy balance of corn ethanol: An update.
United States Department of Agriculture. Agricultural Economic Report
Number 813, 16 pages, 2002.
- R. Wallace, K. Ibsen, A. McAloon and W. Yee.
(2005). Feasibility Study for Co-Locating and Integrating Ethanol
Production Plants from Corn Starch and Lignocellulosic Feedstocks.
US Department of Agriculture and US Department of Energy.
- Ward, O.P., and Singh, A. Bioethanol technology:
developments and perspectives.
Advances in Applied Microbiology 51,
53-80.
- U.S. Department of Energy.
Biofuels & Greenhouse Gas Emissions: Myths versus facts. 2008.
www.eere.energy.gov/biomass/biomass_basics_faqs.html.
Accessed 16th October 2008.
- United Sates Environmental Protection Agency.
Clean Alternative Fuels: Ethanol. 2002.
http://www.eere.energy.gov/afdc/pdfs/epa_ethanol.pdf.
Accessed 16th October 2008.
Referred journal papers
- S.D. Watt, H.S. Sidhu, M.I. Nelson
and A.K. Ray. (2007a)
Analysis of a model for
ethanol production through continuous fermentation.
ANZIAM Journal E (EMAC2007), 49,
C85-C99, 2007.
http://anziamj.austms.org.au/ojs/index.php/ANZIAMJ/article/view/322
.
- S. Watt, H.S. Sidhu, M.I. Nelson,
and A.K. Ray.
Analysis of a
model for ethanol production through continuous fermentation: Ethanol
productivity.
International Journal of Chemical Reactor Engineering,
8: Article A52,
2010.
http://www.bepress.com/ijcre/vol8/A52.
Referred conference papers
- S.D. Watt, H.S. Sidhu, M.I. Nelson
and A.K. Ray. (2007b)
Improving ethanol production through continuous fermentation.
In Proceedings of the 35th Australasian Chemical
Engineering Conference, CHEMECA 2007, pages 1862-1869 (on CDROM),
Engineers Australia, 2007.
ISBN 0-858-25844-7.
- H.S. Sidhu, J. Kavanagh, S.D. Watt and
M.I. Nelson.
Performance Evaluation of Ethanol Production Through
Continuous Fermentation.
In Proceedings of the 36th Australasian Chemical
Engineering Conference, CHEMECA 2008, pages 590-599 (on CDROM),
Engineers Australia, 2008.
ISBN 85825-823-4.
<< Return to my list of projects in
chemical reactor engineering.
<< Return to my list of research
interests.
<< Return to my start page.
Page Created: 19th January 2009.
Last Updated: 10th March 2010.