Professor Mark Wilson Research Information
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Publications
1. Wilson, M R & Walker, N A (1988) The transport and metabolism of urea in Chara australis. I Passive diffusion, specific transport and metabolism of urea, thiourea and methylurea. J. Exp. Bot. 39: 739-751
2. Wilson, M R & Walker, N A (1988) The transport and metabolism of urea in Chara australis. II The urease and the fate of transported urea. J.Exp.Bot. 39: 753-761
3. Wilson, M R, O'Donahue, S I & Walker, N A (1988) The transport and metabolism of urea in Chara australis. III Two specific transport systems. J.Exp.Bot. 39: 763-774
4. Wilson, M R, Hush, J M & Walker, N A (1988) The involvement of microbial epiphytes in uptake measurements in the giant-celled alga Chara australis. Aust.J.Pl.Physiol. 15: 483-494
5. Wilson, M R & Wotherspoon, J S (1988) A new micrsophere-based immunofluorescence assay using flow cytometry. J.Immunol.Methods 107: 225-230
6. Wilson, M R, Mulligan, S P & Raison, R L (1988) A new micrsophere-based immunofluorescence assay for membrane-associated antigens. J.Immunol.Methods 107: 231-237
7. Mckay, D R, Bautovitch, G J, Wilson, M R & Walker, K Z (1989) The effect of circulating antigen on radioimmunodetection and monoclonal antibody localization: studies in a normal rat model. Eur.J.Nucl.Med. 15: 313-320
8. Wilson, M R, O'Donohue, S I & Walker, N A (1990) Urea transport and metabolism in Chara corallina pp. 393-394 In: Membrane Transport in Plants and Fungi. The University of Sydney, 1990
9. Wilson, M R, Crowley, S, Odgers, G A & Shaw, L (1991) Immunofluorescent labelling using covalently linked anti-phycoerythrin antibodies and phycoerythrin polymers. Cytometry 12: 373-377
10. Wilson, M R, Roeth, P J & Easterbrook-Smith, S B (1991) Clusterin enhances the formation of insoluble immune complexes. Biochem.Biophys.Res.Commun. 177: 985-990
11. Easterbrook-Smith, S B, Wilson, M R & Wines, B (1992) RHP is antigenically related to Factor H and binds C1q. Mol.Immunol. 29: 1203-1207
12. Wilson, M R & Easterbrook-Smith, S B (1992) Clusterin binds by a multivalent mechanism to the Fab and Fc regions of IgG. Biochim.Biophys.Acta 1159: 319-326
13. Wilson, M R & Easterbrook-Smith, S B (1993) Enzyme complex Amplification (ECA): a signal amplification method for use in immunoassays. Anal.Biochem. 209: 183-187
14. Wilson, M R, Easterbrook-Smith, S B, Lakins, J & Tenniswood, MPR Mechanism of induction and function of clusterin at sites of cell death. In: Clusterin: Function in Vertebrate Organ Development, Function and Adaption. R G Landes Company Molecular biology Intelligence Unit. Austin, TX, USA.
15. Wilson, M R & Easterbrook-Smith, SB. Clusterin: a protein in search of a function. Today's Life Science 8: 26-31
16) Johnston, J M, Rathmell, S K, Wilson, M R & Easterbrook-Smith, S B. (1996) Use of monoclonal antibodies in ELISA assays. Biochemical Education 24: 50-52
17. Kumar, R, O'Grady, & Wilson, M R. (1996) Production of transforming growth factor by distal respiratory epithelial cells. Int J Exp Path 77: 99-107
18. Robertson, G, Garrick, D, Wilson, M R, Martin, D and Whitelaw, E. (1996) Age-dependent silencing of globin transgenes in the mouse. Nucleic Acid Research 24: 1465-1471
19. Partridge, S R, Baker, S, Walker, M & Wilson, M R (1996) Clusterin, a putative complement regulator, binds to the surface of Staphylococcus aureus isolates. Infection and Immunity 64: 4324-4329
20. Humphreys, D, Hochgrebe, T, Tenniswood, M P, Easterbrook-Smith, S B & Wilson, M R. (1997) Clusterin regulates death signals in L929 cells. Biochemistry 36: 15233-15243
21. Gelissen, I, Hochgrebe, T, Wilson, M, Easterbrook-Smith, S, Jessup, W, Dean, R, & Brown, A. (1998) Apolipoprotein J (clusterin) induces cholesterol export from macrophage-foam cells: a potential anti-atherogenic function. Biochem J 331: 231-237
22. Wilson, M R. (1998) Apoptosis: Unmasking the Executioner. Cell Death Diff. 5: 646-652
23. Wilson, M R. (1988) Apoptosis: Emerging Pathways. Biochem. Cell Biol. 76: 1-11
24. Humphreys, D, Carver, J, Easterbrook-Smith, S B & Wilson, M R. (1999) Clusterin has chaperone-like activity similar to that of small heat-shock proteins. J. Biol. Chem. 274: 6875-6881
25 Humphreys, D & Wilson, MR. (1999) Modes of L929 cell death induced by TNFα and other cytotoxic agents. Cytokine 11: 773-782
26.Hochgrebe, T., Humphreys, D., & Wilson, MR. and Easterbrook-Smith, S. B. (1999) A re-examination of the role of clusterin as a regulator of complement. Exp Cell Res 249: 13-21
27. Gilmore, K & Wilson, MR. (1999) The Use of Chloromethyl-X-Rosamine (Mitotracker Red) to Measure Loss of Mitochondrial Membrane Potential in Apoptotic Cells is Incompatible with Cell Fixation. Cytometry 36: 355-358
28. Wilson, MR and Easterbook-Smith, SB. (2000) Clusterin is a secreted mammalian chaperone. Trends Biochem Sci 25: 95-98
29. Wong, P, Pfeffer, BA, Bernstein, SL, Chambers, M L, Chader, GJ, Zakeri, ZF, Wu, Y Q, Wilson, MR and Becerra, SP (2000) Clusterin protein diversity in the primate eye. Mol Vision 6: 184-191
30. Poon, S, Easterbrook-Smith, SB, Rybchyn, MS, Carver, JA and Wilson, MR. (2000) Clusterin is an ATP-independent chaperone with very broad specificity that stabilizes stressed proteins in a folding-competent state. Biochemistry 39: 15953-15960
31. Gilmore, K J, Quinn, H E and Wilson, M R (2001). Pinocytic loading of cytochrome c into intact cells specifically induces caspase-dependent permeabilization of mitochondria: evidence for a cytochrome c feedback loop. Cell Death Differ 8: 631-639
32. Lakins, J N, Poon, S, Easterbrook-Smith, S B, Carver, J A, Tenniswood, M P R and Wilson, M R (2002) Evidence that clusterin has discrete chaperone and ligand binding sites. Biochemistry 41: 282-291
33. Poon, S, Treweek, T M, Wilson, M R, Easterbrook-Smith, S B and Carver, J A (2002) Clusterin, a unique extracellular chaperone, specifically interacts with slowly aggregating proteins on their off-folding pathway. FEBS Lett 513: 259-266
34. Hatters, D M, Wilson, M R, Easterbrook-Smith, S B and Howlett, G J (2002) Suppression of apolipoprotein C-II amyloid formation by the extracellular chaperone clusterin. Eur. J. Biochem. 269: 2789-2794
35. Jones, R A, Smail, A & Wilson, M R (2002) Detecting mitochondrial permeability transition by confocal imaging of intact cells pinocytically loaded with calcein. Eur. J. Biochem. 269: 3990-3997
36. Poon, S, Rybchyn, M S, Easterbrook-Smith, S B, Carver, J A, Pankhurst, G J and Wilson, M R (2002) Mildly acidic pH activates the extracellular molecular chaperone clusterin. J. Biol. Chem. 277: 39532-39540
37. Jones, R, Cheung, C Y, Black, F E, Zia, J K, Stayton, P S, Hoffman, A S and Wilson, M R (2003) Poly(2-alkylacrylic acid) polymers deliver molecules to the cytosol by pH-sensitive disruption of endosomal vesicles. Biochemical Journal 372: 65-75
38. Carver, J A, Rekas, A, Thorn, D C and Wilson, M R. (2003) Small heat-shock proteins and clusterin: intra- and extracellular molecular chaperones with a common mechanism of action and function? IUBMB Life 55: 661-668
39. Jones, R A, Poniris, M H and Wilson, M R (2004) pDMAEMA is internalised by endocytosis but does not physically disrupt endosomes. J. Controlled Rel. 96: 379-391
40. Dedmon, M M, Christodoulou, J, Wilson, M R and Dobson, C M (2005) Hsp70 inhibits α-synuclein fibril formation via preferential binding to prefibrillar species. J. Biol. Chem. 280: 14733-14740
41. Huber, C, Miele, G, Thielen, C, Wilson, M R, Heinen, E., Xin Fu, Y, and Aguzzi, A (2005) Lymphotoxin-receptor-dependent genes in lymph node and follicular dendritic cell transcriptomes. J. Immunol. 174: 5526-5536
42. Yerbury, J, Rybchyn, M, Easterbrook-Smith, S, Henriques, C and Wilson, M R (2005) The acute phase protein haptoglobin is a mammalian extracellular chaperone with an action similar to clusterin. Biochemistry 44: 10914-10925
43. Thorn, D C, Meehan, S, Sunde, M, Rekas, A, Gras, S L, MacPhee, C E, Dobson, C M, Wilson, M R and Carver, J A (2005) Amyloid fibril formation by bovine milk κ-casein and its inhibition by the molecular chaperones κs- and β-casein. Biochemistry 44: 17027-17036
44. Yerbury, J, Stewart, E, Wyatt, A and Wilson, M R (2005) Quality control of protein folding in extracellular space. EMBO Reports 6: 1131-1136
45. Chapman, T., Wu, X-Y., Barchia, I., Bettelheim, K. A., Driesen, S., Trott, D., Wilson, M R and Chin, J. J-C. (2006) A comparison of virulence gene profiles between Escherichia coli strains isolated from healthy and diarrheic swine. Applied and Environmental Microbiology 72: 4782-4795 (IF 3.818, ranked 19/86)
46. Stewart, EM, Aquilina, JA, Easterbrook-Smith, SB, Murphy-Durland, D, Jacobsen, C, Moestrup, S and Wilson, M R (2007) Effects of glycosylation on the structure and function of the extracellular chaperone clusterin. Biochemistry 46: 1412-1422
47. Nizard, P, Tetley, S, Le Dréan, Y, Watrin, T, Le Goff, P, Wilson, M R and Michel, D. (2007) Stress-induced retro-translocation of clusterin/ApoJ into the cytosol. Traffic 8: 554-565
48. Kumita, JR, Poon, S, Caddy, GL, Hagan, CL, Dumoulin, M, Yerbury, JJ, Stewart, EM, Robinson, CV, Wilson, M R and Dobson, CM. (2007) The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species. J. Mol. Biol. 369: 157-167
49. Yerbury, JJ, Poon, S, Meehan, S, Thompson, B, Kumita, JR, Dobson, CM and Wilson, M R (2007) The extracellular chaperone clusterin influences amyloid formation and toxicity by interacting with pre-fibrillar structures. FASEB J. 21: 2312-2322
50. Wilson, M R, Yerbury, JJ and Poon, S. (2008) "Extracellular molecular chaperones and amyloids" in the book "Heat Shock Proteins in the Brain: Implications for Neurodegenerative Diseases and Neuroprotection" (Springer Publications, Edited by Alexzander Asea and Ian R. Brown). (accepted 21 July 2007)
51. Wilson, M R and Yerbury JJ. (2008) Instant insight: Think outside the cell. Chemical Biology 3: B15
52. Wilson, M R, Yerbury, JJ and Poon, S. (2008) Potential roles of abundant extracellular chaperones in the control of amyloid formation and toxicity. (cover article) Molecular BioSystems 4: 42 - 52
53. French, K, Yerbury, JJ and Wilson, M R (2008) Protease activation of alpha-2-macroglobulin modulates a chaperone-like action with broad specificity. Biochemistry 47: 1176-1185
54. Olsson, M, Wilson, M R, Uller, T, Mott, B, Isaksson, C, Healey, M and Wanger, T. (2008) Free radicals run in lizard families. Biology Letters doi:10.1098/ rsbl.2007.0611
55. Gillen, C, Courtney, HS, Schulze, K, Rohde, M, Wilson, M R, Timmer, AM, Guzman, CA, Nizet, V, Chhatwal, GS and Walker, MJ. (2008) Opacity factor activity and epithelial cell binding by the serum opacity factor protein of Streptococcus pyogenes are functionally discrete. J. Biol. Chem. (accepted 8 Jan 2008)
56. Olsson, M, Wilson, M R, Uller, T, Mott, B, Isaksson, C. (2008) Variation in levels of reactive oxygen species is explained by maternal identity, sex, and body size-corrected clutch size in a lizard. Naturwissenschaften (accepted 16 Oct 2007)
57. Olsson, M, Wilson, M R, Isaksson, C, Uller, T and Mott, B. (2008) Carotenoid intake does not mediate a relationship between reactive oxygen species and bright coloration: experimental test in a lizard. J. Exp. Biol. (accepted 28 Jan 2008)
Research Experience
Urea transport and metabolism in Chara (1982-85) (PhD project)
Affinity purification of a T cell antigen using monoclonal antibodies (1985-86)
Commercial applications of monoclonal antibodies (1986-88)
Development of a biochemical monoclonal antibody facility (1988-90)
Investigation of Clusterin Function (1990-present)
Development of an amplification system for immunoassays
Signal Transduction in Apoptosis (1996-2003)
Discovery of extracellular mechanisms for quality control of protein folding (2004-present)
Research Direction
Our major current research foci are (i) identification of previously unknown chaperones found in human blood, and (ii) discovering mechanisms used by the human body to "sense" and dispose of misfolded extracellular proteins. This work is important because in a variety of serious human diseases (e.g. Alzheimer's disease, type II diabetes) the aggregation of misfolded extracellular proteins underpins cell death and disease pathology.
Recent Grants Obtained:
ARC SPIRT. 2000-2002. $62,400 (with J Chin, R Campbell, & G Eamens). Increasing animal health and disease resilience with the use of probiotics.
ARC Discovery Project Grant (DP0211310) ($140,000) 2002-2004. With S B Easterbrook-Smith. How does clusterin protect cells from stress?
ARC Discovery Project Grant (DP0208752) ($180,000) 2002-2004. With S B Easterbrook-Smith and J A Carver. The basis of recognition and disposal of dysfunctional proteins by clusterin.
ARC LIEF (LE0237427) ($269,000). 2002. Equipment for fluorescence-based cellular analysis. (Co-ordinator with M Ranson).
Ramaciotti Foundations grant, Digital photography equipment for microscopy, M R Wilson & M R Ranson (co-ordinators) ($15,000) 2002.
University of Wollongong Strategic Research Development Grant ($15,000) 2003.
ARC LIEF (LE0453832) $550,910. 2003. New directions in biomolecular mass spectrometry (with 12 others).
Ramaciotti Foundations grant ($30,000). 2004. Multipurpose multiwell plate reader. (Co-ordinator)
ARC (small). ($16,000) 2004. The role(s) of extracellular chaperones in amyloid formation and toxicity.
ARC LIEF (LE0560751) ($432,474) 2004. Facility for the analysis of biomacromolecular interactions (with 13 others).
AINSE Postgraduate Research Award ($39,000; 2005-2008). Disposal of unfolded proteins by members of the LDL receptor superfamily. (PhD student: Amy Wyatt)
URC (small) 2006 Anti-inflammatory effects of the extracellular chaperone clusterin in rheumatoid arthritis. ($16,000)
Alzheimer's Australia Research, Rosemary Loader Research grant ($10,000; 2007). Do the effects of extracellular chaperones on Ab clearance and toxicity provide potential therapeutic targets? J Yerbury and M R Wilson.
UoW UIC International Linkages grant. ($9930). 2006. Building a protein structure/function focussed link with the University of Cambridge.
ARC Discovery-Project grant (DP0773555) ($263,000; 2007-2009). Discovering Mechanisms for Quality Control of Extracellular Protein Folding. M R Wilson and J A Aquilina.
DEST International Scientific Linkages programme competitive grant (CG110083). Building a Protein Folding Disease Focussed Link with the University of Cambridge. M R Wilson, A Aquilina, C V Robinson and C M Dobson. ($41,000; 2007-2009)
UoW URC Small grant. ($13,000) 2008. Identifying new cellular assays for the rapid assessment of environmental algal toxicology. D Jolley & M R Wilson.
ARC Linkage International Fellowship (LX0881953; $92,476, 2008). The effects of alpha-2-macroglobulin on amyloid formation and toxicity. JJ Yerbury and M R Wilson.
Australian Academy of Sciences Scientific Visits to Europe grant-in-aid ($8,100; 2008/9). MR Wilson.
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